Application

HRP conjugated goat anti-mouse gamma chain specific was used to detect neutralizing antibodies against HIV1 in sera and mucosal washings by ELISA. 3′3′5,5 tetramethylbenzidine (Sigma) was used as the substrate for development.

Goat Anti-Mouse IgG (γ-chain specific)-Peroxidase antibody has been used for ELISA applications at a dilution of 1:3000.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.Goat Anti-Mouse IgG (γ-chain specific)-Peroxidase antibody is specific for mouse IgG when tested against purified mouse IgA, IgG, and IgM myeloma proteins.

Immunogen

purified mouse IgG

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).